Native Human Apolipoprotein B (ab91112)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
-
Product name
Native Human Apolipoprotein B
See all Apolipoprotein B proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
-
Species
Human
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab91112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Lyophilized -
Additional notes
Prepared from fresh, non-frozen plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 10.00
Constituents: 0.53% Sodium carbonate, 0.415% Sodium deoxycholate, 0.29% Sodium chloride
General Info
-
Alternative names
- Apo B
- Apo B 100
- Apo B-100
see all -
Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
Images
-
SDS-PAGE: 4-12% Bis-Tris-HCI NuPAGE gel
Lane 1. 5 μg Apolipoprotein (reduced /heated)
Lane 2. 10 μg Apolipoprotein (reduced/ heated)
Lane 3. 20 μg Apolipoprotein (reduced/ heated)
Lane 4. Molecular weight markers
Lane 5. 5 μg Apolipoprotein (non-reduced/no heat)
Lane 6. 10 μg Apolipoprotein (non-reduced/no heat)
Lane 7. 20 μg Apolipoprotein (non-reduced/no heat)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab91112 has not yet been referenced specifically in any publications.