Natural Human Azurocidin protein (ab91129)

Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab91129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Additional notes
    Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    De-ionized H2O

General Info

  • Alternative names
    • Azu 1
    • AZU1
    • Azurocidin
    • CAP37
    • CAP7_HUMAN
    • Cationic antimicrobial protein 37
    • Cationic antimicrobial protein CAP37
    • HBP
    • Heparin binding protein
    • Heparin-binding protein
    • HUMAZUR
    • Neutrophil azurocidin
    see all
  • Function
    This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
  • Sequence similarities
    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Cytoplasmic granule. Cytoplasmic granules of neutrophils.
  • Information by UniProt

Images

  • Anti-Azurocidin antibody (ab48132) at 1 µg/ml + Natural Human Azurocidin protein (ab91129) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes
  • Lane 1. LMW Standard
    Lane 2. Azurocidin - 5 µg (reduced / heated)
    Lane 3. Azurocidin - 10 µg (reduced / heated)
    Lane 4. Azurocidin - 20 µg (reduced / heated)
    4-12% Bis-Tris SDS-PAGE gel

References

ab91129 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. This protein is lyophilised from de-ionized water, and reconstituted to pre-lyophilisation volume with de-ionized water. I hope this information is helpful to you. Please do not hesitate to contact us if you ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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