Natural human Catalase protein (ab91026)

Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab91026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.39. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Cas1
    • CAT
    • CATA_HUMAN
    • Catalase
    • Cs1
    • MGC138422
    • MGC138424
    see all
  • Function
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities
    Belongs to the catalase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Peroxisome.
  • Information by UniProt

Images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 µg Catalase (reduced/heated)
    Lane 2: 10 µg Catalase (reduced/heated)
    Lane 3: 20 µg Catalase (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 µg Catalase (non-reduced/no heat)
    Lane 6: 10 µg Catalase (non-reduced/no heat)
    Lane 7: 20 µg Catalase (non-reduced/no heat)

References

ab91026 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam.

Having talked to the lab, they told me that frozen Catalase has the appearancethat you described. Therefore the vial is not contaminated as I first thought andso you can continue your experiments as normal. Read More

Thank you for contacting Abcam.

I am sorry that the vial of ab91026 was contaminated upon arrival. I have set the product to ship on Monday and it should arrive to you on Tuesday morning. Your new order number is ******.

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