The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivity: Greater than 200 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Z-Arg-Arg-beta-naphthylamide per minute at 40°C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Western blot - Anti-Cathepsin B antibody [CA10] (ab58802)
Anti-Cathepsin B antibody [CA10] (ab58802) at 0.25 µg/ml + Natural human Cathepsin B protein (ab90387) at 0.01 µg
Secondary Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Natural human Cathepsin B protein (ab90387)
This product has been referenced in:
Yoon JY et al. Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J3:e153 (2013).
Read more (PubMed: 24141622) »