The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: 541 Units/mg (prior to lyophilization).
One unit is the amount of enzyme which digests hemoglobin releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1.0 per 60 minutes at 37ºC. Substrate: Acid denatured hemoglobin (0.2% in reaction mixture). Buffer: 0.1 M formate, pH 3.3.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute with 28.3ul distilled water. Protein: 0.883mg/ml (prior to lyophilization).
Cathepsin D heavy chain
Ceroid lipofuscinosis neuronal 10
Epididymis secretory sperm binding protein Li 130P
HEL S 130P
Lysosomal aspartyl peptidase
Lysosomal aspartyl protease
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Expressed in the aorta extrcellular space (at protein level).
Involvement in disease
Ceroid lipofuscinosis, neuronal, 10
Belongs to the peptidase A1 family. Contains 1 peptidase A1 domain.
N- and O-glycosylated.
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).