Natural human Cathepsin D protein (ab91123)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab91123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesProtein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.0 Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.

    Preservative: None
    Constituents: 2mM Sodium phosphate, pH 6.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionResuspend a 25ug vial with 71.4uL of deionized H2O to give a protein concentration of 0.350mg/mL, which is the original protein concentration of the lot when it was finished. This should then be aliquotted into suitable amounts in a screw top vial to prevent sublimation and stored at <70 deg C. Aliquots should be diluted to appropriate concentration once thawed. We recommend 2mM NaP pH 6.5 to make dilutions.

General Info

  • Alternative names
    • CatD
    • CATD_HUMAN
    • Cathepsin D heavy chain
    • CD
    • Ceroid lipofuscinosis neuronal 10
    • CLN10
    • CPSD
    • ctsd
    • Epididymis secretory sperm binding protein Li 130P
    • HEL S 130P
    • Lysosomal aspartyl peptidase
    • Lysosomal aspartyl protease
    • MGC2311
    see all
  • FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Tissue specificityExpressed in the aorta extrcellular space (at protein level).
  • Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
  • Sequence similaritiesBelongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt

Natural human Cathepsin D protein images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1. Molecular weight markers
    Lane 2. 4 µg Cathepsin D (non-reduced/no heat)
    Lane 3. 7 µg Cathepsin D (non-reduced/no heat)
    Lane 4. Molecular weight markers
    Lane 5. 4 µg Cathepsin D (reduced/heated)
    Lane 6. 7 µg Cathepsin D (reduced/heated)

References for Natural human Cathepsin D protein (ab91123)

ab91123 has not yet been referenced specifically in any publications.

Product Wall

One unit of activity is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A 280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, p...

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As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial of ab66587 has been added to your...

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I have discussed this case with the originator of this protein. I can confirm there are no reports of any problems with this lot. They have also confirmed that this antibody i...

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The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our record...

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Thank you for your enquiry.

I can confirm that the reconstitution of ab91123Cathepsin D protein (Active)is lot dependent. I have reviewed your orders, and have found order number #####, and the lot number from this order is GR35865-3. Could ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"