The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivity: Greater than 1 unit per mg of protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Z-Phe-Arg-AFC per minute at 25°C using 400 mM Na Acetate, pH 5.5 with 4 mM EDTA and 8 mM DTT as the activation buffer in the presence of Brij.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.