Natural Human Ceruloplasmin protein (ab77930)

Overview

  • Product nameNatural Human Ceruloplasmin protein
  • Protein lengthFull length protein

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab77930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesAll human source materials have tested negative for HIV-1 and HIV-2 and non-reactive with HCV and HBc antibodies and HBsAg. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.

    Storage Notes: Prolonged exposure to light will degrade the ceruloplasmin. This will lead to a decrease in the blue color of the product.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

    Preservative: None
    Constituents: 50mM Potassium phosphate, 5mM EDTA, 20mM EACA, 100mM Potassium chloride, pH 6.8

  • ReconstitutionReconstitute with distilled water. Please contact Abcam for lot-specific recommended volume.

General Info

  • Alternative names
    • CERU_HUMAN
    • Ceruloplasmin
    • CP
    • CP 2
    • CP2
    • Ferroxidase
    see all
  • FunctionCeruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • Sequence similaritiesBelongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt

Natural Human Ceruloplasmin protein images

  • Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml + Natural Human Ceruloplasmin protein (ab77930) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Natural Human Ceruloplasmin protein (ab77930)

ab77930 has not yet been referenced specifically in any publications.

Product Wall

For ab77930 Human Ceruloplasmin protein, the copper ions are stably bound as long as you do not change the buffer. The buffer this was lyophilized from is as follows:

Buffer: Lyophilized from 50 mM Potassium Phosphate, pH 6.8, 100 mM Potassi...

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Thank you for contacting us.
We unfortunately haven't tested this product for its enzymatic activity so I am sorry we are unable to confirm that the product can act as enzyme. The activity might have to be determined by end user.
This product ...

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Gracias por tu respuesta.
Efectivamente me refería a 140uL en lugar de 140 ml, siento la confusión.
Es posible diluir en mayor volumen para obtener una concentración menor, pero hay que tener en cuenta que al diluir la proteína también se dil...

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Muchas gracias por contactarnos. Te pido disculpas por la espera.
La concentración de este lote antes de la liofilización era de 7.137mg/mL. Para reconstituirlo, al vial de 1mg se le deben añadir 140 ml de agua destilada.
Espero haber resuelt...

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Gracias por su respuesta.
Le confirmo que el kit ab102850 para conjugar la proteína Ceruloplasmin con fosfatasa alcalina es válido.
La oferta del 4 por 3 sigue vigente siempre que nos indique el código de referencia de dicha promoción en el p...

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Thank you for contacting us.

Lot number GR70342-1 is at 7.137mg/mL. A 1mg vial can be reconstituted using 140uL distilled water. This will return the 1mg vial to the original protein concentration and buffer composition.

I hope this...

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Thank you for your new enquiry regarding ab77930. GR44819 is the same as batch: 1104116: Please reconstitute a 1mg vial with 310uL of water.  This will return the product to the original protein concentration of 3.23mg/mL and to the original buffer...

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Thank you for your enquiry regarding ab77930. The concentration before lyophilization is lot-specific (each lot can have a different concentration). The label of the vial should indicate the actual concentration. Batch 699123: Reconstitute a 1m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"