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Our Abpromise guarantee covers the use of ab98981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Starting material serum donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.
IgG from pooled normal human serum/plasma
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.
Constituents: 0.02M Ammonium bicarbonate
(May contain traces of buffer salts)
The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab20149 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
ab98981 has not yet been referenced specifically in any publications.