• Nature
  • Source
  • Amino Acid Sequence
    • Species


Our Abpromise guarantee covers the use of ab90286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.
  • Applications


  • Purity
    > 95 % SDS-PAGE.

  • Form
  • Additional notes

    IgG4 from human myeloma plasma. MW: 146,000.

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm =1.36.

    Shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.05% Sodium Azide
    Constituents: 20mM PBS, 150mM Sodium chloride, pH 7.4

General Info

  • Alternative names
    • Ig gamma 4 chain C region
    • IGHG4
    • immunoglobulin heavy chain constant region gamma 4
    • Immunoglobulin heavy constant gamma 4 (G4m marker)
    • MGC117419
    see all
  • Relevance
    IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.
  • Cellular localization


  • SDS-PAGE analysis of Active human IgG4 full length protein (ab90286).

    1. IgG4 - 5 μg (reduced / heated)

    2. IgG4 - 10 μg (reduced / heated)

    3. IgG4 - 20 μg (reduced / heated)

    4. IgG4 - 5 μg (non-reduced / no heat)

    5. IgG4 - 10 μg (non-reduced / no heat)

    6. IgG4 - 20 μg (non-reduced / no heat)


This product has been referenced in:
  • Vidal M  et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). Read more (PubMed: 29397157) »
  • Masat E  et al. Long-term exposure to Myozyme results in a decrease of anti-drug antibodies in late-onset Pompe disease patients. Sci Rep 6:36182 (2016). Read more (PubMed: 27812025) »

See all 2 Publications for this product

Customer reviews and Q&As

Neither of these proteins are recombinant. They are isolated from myeloma plasma, identified as IgG1 and IgG4 myelomas.

These products are tested for possible contaminating IgGs by ELISA. Each of these products is at least 95% pure and by ELI...

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IgG4 is a complete immunoglobulin in that it has 2 heavy chains and 2 light chains per molecule. However, this is a myeloma which means that every molecule contains either Lambda Light chain or Kappa Light Chain; not both like would be found in a ...

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Vielen Dank für Ihren Anruf.

Ich habe nun die Antwort aus dem Labor erhalten, und Sie sollten ohne Probleme dieses Produkt als Positivekontrolle im ELISA einsetzen können:

"Yes, you may use this product as a positive...

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Thank you for contacting us.

The product ab90286 has kappa light chain however ab98893 has lamda light chain.

The proteins are dissolved in different storage buffer and the concentration is different.

These product are from ...

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Thank you for contacting us.

The protein has a Kappa Light Chain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abrevie...

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Thank you for your email.

I am happy to check these concentrations for you. Please find here below the answers:

IgG1 protein (ab90283) you will receive 1 mg at 3.41mg/ml, ¨so it will be in 293ul

IgG2 protein...

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Thank you for contacting us with your question.

We don't assign specific expiration dates to our products (most haven't been tested for an exact date) but we are able to guarantee the protein for up to 6 months after purchase.

I hop...

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Vielen Dank für Ihre Anfrage.

Da es sich hier um ein inaktives Protein handelt, kann es bis zu einer Woche bei 4C gelagert werden. Da dem Protein kein Glycerin zugesetzt wurde, sollte eine Lagerung bei -40 oder -80C nichts im Wege...

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Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies and proteinsare stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follo...

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Thank you for contacting us.

Unfortunately this is not a product that we carry at this time. The Fc region may be cleaved from F(ab) region with papain or pepsin and purified out but this assay would have to be worked out experimentally. Read More

1-10 of 12 Abreviews or Q&A


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