Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman

Associated products

Specifications

Our Abpromise guarantee covers the use of ab91115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.
    Purity: single arc by IEP against antisera to whole human serum. Essentially free of other plasma lipoproteins as determined by electrophoresis using Fat Red 7B stain for lipids and Coomassie Blue for proteins. >=95% of total lipoprotein content by electrophoresis.
  • FormLiquid
  • Additional notesPrepared from fresh, non-frozen plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.

    Preservative: None
    Constituents: 0.01% EDTA, 150mM Sodium chloride, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • low density lipoprotein
  • RelevanceThe low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis (1). Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits (2). It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) was shown to be mediated by LDL receptors on cultured cells
  • Cellular localizationCell Membrane

Natural human LDL protein images

  • SDS-PAGE: 12% Tris HCl gel
    10 µg LDL (reduced / heat)

References for Natural human LDL protein (ab91115)

ab91115 has not yet been referenced specifically in any publications.

Product Wall

Application Other

Review text: RAW264.7 cells (0.5 million cells per well in 24 well plate) were stimulated with LDL protein (in different doses between 10ug/ml and 30 ug/ml) for 24 hours and after harvesting by scraping, cells were scored for surface expression of specific cell surface scavenging receptor CD36 by flow cytometry. Anti-Mouse CD36 APC primary antibody conjugate was used for staining in flow cytometry.
Sample: Mouse Cultured Cells

Primary antibody (in addition to 'LDL protein (Active)')
Name: Non-Abcam antibody was used: anti-Mouse CD36 APC
Host species: Rat
Clonality: Monoclonal
Conjugation: Allophycocyanin
Concentration: 0.06 µg/10^6 cells

Secondary antibody
Secondary antibody: None used
Username

Mr. Pijus Barman

Verified customer

Submitted Jun 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"