Natural Human LMW Kininogen protein (ab91118)


  • Product nameNatural Human LMW Kininogen protein
  • Protein lengthFull length protein


  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman


Our Abpromise guarantee covers the use of ab91118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


    Western blot

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesPrepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Preservative: None
    Constituents: 200mM Sodium chloride, 10mM Sodium acetate, pH 5.5

General Info

  • Alternative names
    • Alpha-2-thiol proteinase inhibitor
    • BDK
    • BK
    • Fitzgerald factor
    • High molecular weight kininogen
    • HMWK
    • Ile-Ser-Bradykinin
    • Kallidin I
    • Kallidin II
    • Kininogen 1
    • KNG
    • KNG1
    • KNG1_HUMAN
    • LMW
    • Low molecular weight growth-promoting factor
    • Low molecular weight kininogen
    • Williams-Fitzgerald-Flaujeac factor
    see all
  • Function(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • Tissue specificitySecreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • Involvement in diseaseDefects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
  • Sequence similaritiesContains 3 cystatin kininogen-type domains.
  • Post-translational
    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracellular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt

Natural Human LMW Kininogen protein images

  • Anti-LMW Kininogen antibody (ab79650) at 1 µg/ml + Natural Human LMW Kininogen protein (ab91118) at 0.01 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • Lane 1 : Anti-LMW Kininogen antibody (ab79650) at 1/100 dilution
    Lane 2 : Coomassie Blue staining

    Lane 1 : Natural Human LMW Kininogen protein (ab91118)
    Lane 2 : Natural Human LMW Kininogen protein (ab91118)

    Lysates/proteins at 0.01 µg per lane.

    HRP-conjugated goat anti-rabbit monoclonal at 1/1000 dilution
    developed using the ECL technique

    Exposure time : 20 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Natural Human LMW Kininogen protein (ab91118)

ab91118 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot

Sample: Human Purified protein (Purified LMW Kininogen diluted in Laemmli buffer)
Loading amount: 0.01 µg
Specification: Purified LMW Kininogen diluted in Laemmli buffer
Gel Running Conditions: Reduced Denaturing (12%)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details
Incubation time: 15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk

Primary antibody (in addition to 'LMW Kininogen protein (Human)')
Dilution: 1/100

Secondary antibody
Name: Non-Abcam antibody was used: goat anti-rabbit IgG-HRP,Santa Cruz biotechnology
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/1000

Detection method: Thermo scientific super-signal west pico
Exposure: 20 minute(s) and 0 second(s)
Bands: Specific: 68 kDa
Positive control: Coomassie Blue Staining of 1D gel was performed prior WB application

Additional data
Additional Notes: Western blot - LMW Kininogen purified protein ab91118. The blot is stained with ab79650, Anti LMW Kininogen antibody, rabbit polyclonal.
Coomasie Blue stain - LMW Kininogen purified protein ab91118.

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Submitted Jun 29 2012