Natural Human LMW Kininogen protein (ab91118)

Overview

  • Product name
    Natural Human LMW Kininogen protein
  • Protein length
    Full length protein

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab91118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Additional notes
    Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Preservative: None
    Constituents: 200mM Sodium chloride, 10mM Sodium acetate, pH 5.5

General Info

  • Alternative names
    • Alpha-2-thiol proteinase inhibitor
    • BDK
    • BK
    • Fitzgerald factor
    • High molecular weight kininogen
    • HMWK
    • Ile-Ser-Bradykinin
    • Kallidin I
    • Kallidin II
    • Kininogen 1
    • KNG
    • KNG1
    • KNG1_HUMAN
    • LMW
    • Low molecular weight growth-promoting factor
    • Low molecular weight kininogen
    • Williams-Fitzgerald-Flaujeac factor
    see all
  • Function
    (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • Tissue specificity
    Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • Involvement in disease
    Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
  • Sequence similarities
    Contains 3 cystatin kininogen-type domains.
  • Post-translational
    modifications
    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracellular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt

Images

  • Anti-LMW Kininogen antibody (ab79650) at 1 µg/ml + Natural Human LMW Kininogen protein (ab91118) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • Lane 1 : Anti-LMW Kininogen antibody (ab79650) at 1/100 dilution
    Lane 2 : Coomassie Blue staining

    Lane 1 : Natural Human LMW Kininogen protein (ab91118)
    Lane 2 : Natural Human LMW Kininogen protein (ab91118)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit monoclonal at 1/1000 dilution
    Developed using the ECL technique

    Exposure time : 20 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References

ab91118 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot

Sample: Human Purified protein (Purified LMW Kininogen diluted in Laemmli buffer)
Loading amount: 0.01 µg
Specification: Purified LMW Kininogen diluted in Laemmli buffer
Gel Running Conditions: Reduced Denaturing (12%)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details
Incubation time: 15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk

Primary antibody (in addition to 'LMW Kininogen protein (Human)')
Dilution: 1/100

Secondary antibody
Name: Non-Abcam antibody was used: goat anti-rabbit IgG-HRP,Santa Cruz biotechnology
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/1000

Detection
Detection method: Thermo scientific super-signal west pico
Exposure: 20 minute(s) and 0 second(s)
Bands: Specific: 68 kDa
Positive control: Coomassie Blue Staining of 1D gel was performed prior WB application

Additional data
Additional Notes: Western blot - LMW Kininogen purified protein ab91118. The blot is stained with ab79650, Anti LMW Kininogen antibody, rabbit polyclonal.
Coomasie Blue stain - LMW Kininogen purified protein ab91118.
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Submitted Jun 29 2012

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