Natural human Myeloperoxidase protein (ab91116)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab91116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: 180-220 units per mg protein after lyophilization. One unit of activity is defined as the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 6.1. Reaction mixture contains 33 mM sodium phosphate, pH 6.1, 30 mM guaiacol, and 0.0012% (0.35 mM) hydrogen peroxide.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesProtein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.45. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Preservative: None
    Constituents: 50mM Sodium acetate, 100mM Sodium chloride, pH 6.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 84 kDa myeloperoxidase
    • 89 kDa myeloperoxidase
    • EC 1.11.1.7
    • EC1.11.2.2
    • fj80f04
    • MPO
    • mpx
    • myeloid-specific peroxidase
    • Myeloperoxidase
    • Myeloperoxidase heavy chain
    • Myeloperoxidase light chain
    • PERM_HUMAN
    • wu:fj80f04
    see all
  • FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
  • Cellular localizationLysosome.
  • Information by UniProt

Natural human Myeloperoxidase protein images

  • SDS-PAGE: 4-12% Bis-Tris gel
    Lane 1. 5 µg Myeloperoxidase (reduced/heated)
    Lane 2. 10 µg Myeloperoxidase (reduced/heated)
    Lane 3. 20 µg Myeloperoxidase (reduced/heated)
    Lane 4. Molecular weight markers

References for Natural human Myeloperoxidase protein (ab91116)

ab91116 has not yet been referenced specifically in any publications.

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