Natural human Neutrophil Elastase protein (ab91099)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Molecular weight29500 kDa

Specifications

Our Abpromise guarantee covers the use of ab91099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: 20-22 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Meo-suc-ala-pro-val-pNA per minute at 25°C in 100 mM Tris-HCl, pH 7.5, with 500 mM NaCl.
  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesReconstitute in 50 mM Na acetate pH 5.5 with 150 mM NaCl. Dissolve at a concentration of 1 to 2 mg/mL and store at -20 degrees C. If not immediately frozen, this enzyme should be used promptly to limit loss of activity. We do not suggest repeated thawing/freezing cycles. Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.985. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Preservative: None
    Constituents: ddH20

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Bone marrow serine protease
    • ELA2
    • ELANE
    • Elastase 2
    • Elastase 2 neutrophil
    • Elastase neutrophil expressed
    • Elastase-2
    • ELNE_HUMAN
    • GE
    • Granulocyte derived elastase
    • HLE
    • HNE
    • Human leukocyte elastase
    • Leukocyte elastase
    • Medullasin
    • NE
    • Neutrophil elastase
    • PMN E
    • PMN elastase
    • Polymorphonuclear elastase
    • SCN1
    see all
  • FunctionModifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
  • Tissue specificityBone marrow cells.
  • Involvement in diseaseDefects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
    Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
  • Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt

Natural human Neutrophil Elastase protein images

  • SDS-PAGE: 12% Bis-Tris NuPAGE gel
    Lane 1: 5 µg Neutrophil Elastase (reduced/heated)
    Lane 2: 10 µg Neutrophil Elastase (reduced/heated)
    Lane 3: 20 µg Neutrophil Elastase (reduced/heated)
    Lane 4: Molecular weight markers

References for Natural human Neutrophil Elastase protein (ab91099)

ab91099 has not yet been referenced specifically in any publications.

Product Wall

We only have 3 active human neutrophilase elastase proteins in our catalogue:

http://www.abcam.com/active-human-neutrophil-elastase-full-length-protein-ab91099.html
http://www.abcam.com/active-human-neutrophil-elastase-full-length-protein-...

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Thank you for contacting us.

As a follow up from our phone call, the lab has confirmed that this protein has not been tested for endotoxins.


I hope this information is helpful to you. Please do not hesitate to contact us if ...

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Thats no problem. If you have any further questions, please do not hesitate to contact us again.

I now have some further information to explain the 14 kDa band observed in both the SDS page gel and Western blot of Neutrophil Elastase protein (Active), ab91099.

I have been informed by the lab that performing SDS-page under reducing condi...

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I understand your concerns in regards to this second band. From the SDS-page gel, it appears that the band at ˜14 kDa also increases with increasing concentration (it is quite low in the 5 ug lane). It therefore appears that the band is concentration d...

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Thank you for providing me with that extra information.
I now have some more information to share with you. It seems that the unexpected band at 14 kDa is more likely to be observed in the western blot if the sample is overloaded with protein. As ...

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Thank you for providing that information. I now understand more fully what your concerns regarding the protein are.
It seems that the lower band is much more prominent (in comparison to the expected ˜25 kDa band) when blocked with BSA and a higher...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"