Natural human Plasma Kallikrein 1B protein (ab77870)

Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab77870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: >=15 units per mg. One unit is defined as the amount of enzyme that will hydrolyze 1µmole of D-Pro-Phe-Arg-pNA (Chromogenix S-2303) per minute at 25°C, pH 7.8.
  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM Tris HCl, 100mM Sodium chloride, pH 7.8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with 104ul distilled water to get a 0.481 mg/ml solution. (0.3mg/ml (prior to lyophilization).)

General Info

  • Alternative names
    • Fletcher factor
    • kallikrein B plasma
    • Kallikrein B plasma (Fletcher factor) 1
    • Kallikrein B, plasma 1
    • Kallikrein B1
    • Kininogenin
    • KLK3
    • KLKB1
    • KLKB1_HUMAN
    • PKK
    • PKKD
    • Plasma kallikrein
    • Plasma kallikrein B1
    • Plasma kallikrein light chain
    • Plasma prekallikrein
    • PPK
    • Prekallikrein
    see all
  • Function
    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Involvement in disease
    Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
  • Sequence similarities
    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt

References for Natural human Plasma Kallikrein 1B protein (ab77870)

ab77870 has not yet been referenced specifically in any publications.

Product Wall

The source of the Plasma Kallikrein 1B protein ab77870 informed me that the purification method is proprietary information and cannot be provided.

Sorry we can't be more helpful on this occasion.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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