Natural Human Serum Albumin protein (Biotin) (ab8033)



  • Nature
  • Source
  • Amino Acid Sequence
    • Accession
    • Molecular weight
      69 kDa
    • Additional sequence information
      Biotin conjugated
  • Conjugation


Our Abpromise guarantee covers the use of ab8033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Dot blot



    Western blot

  • Form
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2

  • Reconstitution
    Restore with 1.0 ml of deionized water (or equivalent).aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Stabiliser: 10 mg/ml Polyethylene Glycol (PEG-8000) Preservative: 0.01% (w/v) Sodium Azide

General Info

  • Alternative names
    • alb
    • Albumin
    • Albumin (32 AA)
    • Albumin (AA 34)
    • Analbuminemia
    • Bisalbuminemia
    • Cell growth inhibiting protein 42
    • DKFZp779N1935
    • Dysalbuminemic hyperthyroxinemia
    • Growth inhibiting protein 20
    • HSA
    • Hyperthyroxinemia dysalbuminemic
    • PRO0883
    • PRO0903
    • PRO1341
    • Serum albumin
    see all
  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
  • Involvement in disease
    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization
  • Information by UniProt

References for Natural Human Serum Albumin protein (Biotin) (ab8033)

ab8033 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The spacer arm is biotin-epsilon-aminocaproic acid (BAC). Here is a URL with a picture of it:

I hope this information is...

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Thank you for contacting Abcam.

The ratio for this particular lot is 2.5 moles of biotin per mole of human albumin.
Please tell us if we can provide more help with this issue.

Thank you for contacting Abcam regarding ab8033.

I have confirmed with the laboratory that our target biotin to protein ratio is between 10-20 molecules of biotin to protein. I can provide more specific information for a particular lot....

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Thank you for your enquiry. The exact biotin group added was Biotinamidocaproate N-Hydroxysuccinimide Ester. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Yes, you are correct. I am sorry if there was some confusion from my earlier response. ab8033 is not an antibody but rather biotin-labeled albumin. Yes, the molecule has been randomly labeled with biotin at Lys residues. Thank you for your enquir...

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I have a response from our supplier and I want to thank you for your patience. The biotin is attached to the antibody via free primary amines, i.e. at Lys residues. If you have any further questions please do not hesitate to contact me.


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