Anti-Natural Killer Cell Activation Structures antibody [ANK61] (ab36392)

Overview

  • Product nameAnti-Natural Killer Cell Activation Structures antibody [ANK61]
  • Description
    Mouse monoclonal [ANK61] to Natural Killer Cell Activation Structures
  • SpecificityThe antigen recognized by ab36392 is highly expressed on freshly isolated and cultured NK cells. The antibody also binds to rat alpha beta TCR T cells and at a low level to B cells.
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Cell preparation of IL 2 activated cultured NK cells (Wag rats)

  • General notes


    Triggering of the antigen by ab36392 antibody activates the lytic machinery of rat NK cells, but not of T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: Tissue culture supernatant
  • PurityTissue culture supernatant
  • Primary antibody notesTriggering of the antigen by ab36392 antibody activates the lytic machinery of rat NK cells, but not of T cells.
  • ClonalityMonoclonal
  • Clone numberANK61
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Use at an assay dependent concentration.

IHC-P Use at an assay dependent concentration.

Use at an assay dependent concentration.

Target

  • RelevanceNatural killer cells (NK) are a type of lymphocyte and a component of innate immune defense. They share a common progenitor with T cells and have been described as large, granular, bone-marrow derived lymphocytes. These cells do not destroy the attacking microorganisms directly; they attack infected cells and cells that appear that they might be cancer. NK cells are not phagocytic; they weaken the target cell's plasma membrane, causing water and ions to diffuse into the cell and expanding it. Under this large pressure, the target cell lyses. NK cells are characterised immunohistochemically by the presence of CD56 and the absence of CD3 on the cell membrane. NK cells are activated in response to interferons or macrophage-derived cytokines. They serve to contain virus infections while the adaptive immune response is generating antigen specific cytotoxic T cells that can clear the infection. Patients deficient in NK cells prove to be highly susceptible to early phases of herpes virus infection.

References for Anti-Natural Killer Cell Activation Structures antibody [ANK61] (ab36392)

ab36392 has not yet been referenced specifically in any publications.

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There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections ca...

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The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections
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It does look like we changed the storage instructions of ab2557. Since this product was tested in a guaranteed species and technique and she wasn't seeing any staining, we could offer a free of charge replacement ord...

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Thank you forhaving your customercomplete our questionnaire. I am sorry to hear that these antibodies are not providing satisfactory results for them. The details provided will enable us to investigate this case and will provide us with vital informati...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"