Anti-Natural Killer Cell antibody [ANK44] (ab36388)

Overview

  • Product name
    Anti-Natural Killer Cell antibody [ANK44]
  • Description
    Mouse monoclonal [ANK44] to Natural Killer Cell
  • Specificity
    ab36388 also binds to rat gamma-delta-TCR T cells. It does not bind to alpha-beta-TCR T cells or to B cells. The antigen recognized by ab36388 is highly expressed on rat NK cells after IL-2-activation. The antigen is not expressed by unstimulated NK cells.
  • Tested applications
    Suitable for: IHC-Fr, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Preparation of IL-2-activated cultured NK cells (Wag rats)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    ANK44
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Natural killer cells (NK) are a type of lymphocyte and a component of innate immune defense. They share a common progenitor with T cells and have been described as large, granular, bone-marrow derived lymphocytes. These cells do not destroy the attacking microorganisms directly; they attack infected cells and cells that appear that they might be cancer. NK cells are not phagocytic; they weaken the target cell's plasma membrane, causing water and ions to diffuse into the cell and expanding it. Under this large pressure, the target cell lyses. NK cells are characterised immunohistochemically by the presence of CD56 and the absence of CD3 on the cell membrane. NK cells are activated in response to interferons or macrophage-derived cytokines. They serve to contain virus infections while the adaptive immune response is generating antigen specific cytotoxic T cells that can clear the infection. Patients deficient in NK cells prove to be highly susceptible to early phases of herpes virus infection.
  • Alternative names
    • Large granular lymphocytes antibody
    • LGLs antibody
    • NK cells antibody

References

This product has been referenced in:
  • Giezeman-Smits KM  et al. Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells. J Leukoc Biol 63:209-15 (1998). Read more (PubMed: 9468279) »

See 1 Publication for this product

Customer reviews and Q&As

Following up my previous message, I found the original article describing the production and characterization of the natural killer cell antibody ab36388, clone ANK44. The paper suggests that the protein this antibody recognizes, or at least the epitop...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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