Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesMouse

Specifications

Our Abpromise guarantee covers the use of ab95260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity is determined in a one-stage clotting assay.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab95260 is prepared from highly purified factor IX by activation with factor XIa. The factor IXa is further purified by gel filtration, followed by immunoaffinity purification. Purity is assessed by SDS-PAGE analysis.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    Preservative: None
    Constituents: 50% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Christmas factor
    • Coagulant factor IX
    • Coagulation factor IX
    • Coagulation factor IXa heavy chain
    • F9
    • F9 DEFICIENCY
    • FA9_HUMAN
    • Factor 9
    • Factor IX
    • FIX
    • Plasma thromboplastin component
    • PLASMA THROMBOPLASTIN COMPONENT DEFICIENCY
    • PTC
    see all
  • FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
  • Tissue specificitySynthesized primarily in the liver and secreted in plasma.
  • Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
    Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
    Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
  • Post-translational
    modifications
    Activated by factor XIa, which excises the activation peptide.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Natural mouse Factor IXa protein (ab95260)

ab95260 has not yet been referenced specifically in any publications.

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