The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivity is measured in a Factor Xa clotting assay and/or chromogenic substrate assay.
% SDS-PAGE. ab95261 is prepared by activating purified factor X with the factor X activator isolated from Russell's viper venom. Factor Xa is purified from the activation mixture by chromatography over benzamidine-Sepharose. Purity is determined by SDS-PAGE analysis.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituents: 50% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Activated factor Xa heavy chain
Coagulation factor X
Stuart Prower factor
FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificityPlasma; synthesized in the liver.
Post-translational modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium. N- and O-glycosylated. The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway). The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.