Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesMouse

Specifications

Our Abpromise guarantee covers the use of ab95261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity is measured in a Factor Xa clotting assay and/or chromogenic substrate assay.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab95261 is prepared by activating purified factor X with the factor X activator isolated from Russell's viper venom. Factor Xa is purified from the activation mixture by chromatography over benzamidine-Sepharose. Purity is determined by SDS-PAGE analysis.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    Preservative: None
    Constituents: 50% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Activated factor Xa heavy chain
    • Coagulation factor X
    • F10
    • FA10_HUMAN
    • FX
    • FXA
    • Prothrombinase
    • Stuart factor
    • Stuart Prower factor
    • Stuart-Prower factor
    see all
  • FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • Tissue specificityPlasma; synthesized in the liver.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Natural mouse Factor Xa protein (ab95261)

ab95261 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95261.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"