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Our Abpromise guarantee covers the use of ab155708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In situ hybridization
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
There are primarily two methods for coating a surface with Mussel Adhesive protein: hand-spreading and adsorption. In general, adsorption is recommended because it is more consistent and convenient than hand-spreading. But hand-spreading has its place for special situations.
Hand-spreading: The Mussel Adhesive protein can be deposited on a glass or plastic surface by mechanical spreading. Using a handy tool, such as a glass rod or micropipette tip, microliter volumes of Mussel Adhesive protein in 1% acetic acid can be spread in a thin liquid film. As the acetic acid evaporates, a coating of Mussel Adhesive protein is left behind. After washing with ethanol and water, the vessels are ready to use. Although hand-spreading gives a less uniform coating than adsorption, it may be useful in special situations, for example when only a portion of a dish or glass slide is to be coated
Adsorption: The simplest and most cost-effective method of applying Mussel Adhesive protein is by adsorption from a neutral solution. The method is based on the observation that Mussel Adhesive protein comes out of solution as the pH is raised and spontaneously adsorbs to the first surface it contacts.The resulting coating is quite thin (probably close to a protein monolayer) and more uniform than that achieved by hand-spreading. The major advantages of this adsorption method are: coating vessels of any shape is simple; less MAP is required per cm2 of surface area; greater flexibility - the initial concentrations and adsorption times can be adjusted to best suit.
Shipped at 4°C. Store at +4°C.
Constituent: 1% Acetic acid
ab155708 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"