Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • AccessionP00761
    • SpeciesPig
    • SequenceFPTDDDDKIV GGYTCAANSI PYQVSLNSGS HFCGGSLINS QWVVSAAHCY KSRIQVRLGE HNIDVLEGNE QFINAAKIIT HPNFNGNTLD NDIMLIKLSS PATLNSRVAT VSLPRSCAAA GTECLISGWG NTKSSGSSYP SLLQCLKAPV LSDSSCKSSY PGQITGNMIC VGFLEGGKDS CQGDSGGPVV CNGQLQGIVS WGYGCAQKNK PGVYTKVCNY VNWIQQTIAA N
    • Molecular weight24 kDa
    • Amino acids1 to 231

Specifications

Our Abpromise guarantee covers the use of ab101090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity Approximately 5000 Units/mg material.
    The spectrophotometric assay to determine the activity of trypsin measures the increase in absorbance at 253 nm due to N-a-benzoyl arginine as it is cleaved from N-a-benzoyl arginine ethyl ester, with the concomitant formation of ethanol.
  • FormLyophilised
  • Additional notes


    Trypsin catalyzes the cleavage of peptide bonds in proteins at the carboxyl side of arginine and lysine residues, except when either is followed by a proline residue. Trypsin is commonly used to remove adherent cells during cell culture manipulations carried out in vitro.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionThis product should ideally be re-desiccated under vacuum over silica gel. Dissolves readily at 1mg/mL in 1 mM Hydrochloric acid, can be used in 67mM potassium phosphate buffer to assay for trypsin activity

General Info

  • Alternative names
    • Alpha-trypsin chain 2
    • Anionic trypsinogen
    • Beta trypsin
    • Beta-trypsin
    • Brain trypsinogen
    • Cationic trypsinogen
    • Digestive zymogen
    • Mesotrypsin
    • Mesotrypsinogen
    • MGC111183
    • MGC120174
    • MGC120175
    • MGC149362
    • MTG
    • Nonfunctional trypsin 1
    • Pancreatic trypsinogen III
    • Protease Serine 1
    • Protease serine 1 (trypsin 1)
    • Protease serine 2
    • Protease serine 2 (trypsin 2)
    • Protease serine 2 preproprotein
    • Protease serine 3
    • Protease serine 3 (mesotrypsin)
    • Protease serine 4 (trypsin 4 brain)
    • Prss1
    • PRSS2
    • PRSS3
    • PRSS4
    • Serine protease 1
    • Serine protease 2
    • Serine protease 3
    • Serine protease 4
    • T9
    • TRP1
    • TRY1
    • TRY1_HUMAN
    • TRY2
    • TRY3
    • TRY4
    • TRY8
    • TRYP1
    • TRYP2
    • Trypsin 1
    • Trypsin 2
    • Trypsin 3
    • Trypsin I
    • Trypsin II
    • Trypsin III
    • Trypsin IV
    • Trypsinogen 1
    • Trypsinogen 2
    • Trypsinogen 4
    • Trypsinogen 5
    • Trypsinogen A
    • Trypsinogen, anionic
    • Trypsinogen, cationic
    • Ttrypsinogen IV
    see all
  • FunctionHas activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
  • Involvement in diseaseDefects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt

References for Natural pig Trypsin protein (ab101090)

ab101090 has not yet been referenced specifically in any publications.

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