Natural Protein A (FITC) (ab7455)

Overview

Description

  • NatureNatural
  • SourceNative
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Specifications

Our Abpromise guarantee covers the use of ab7455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Flow Cytometry

    Immunomicroscopy

  • FormLiquid
  • Additional notesMolecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2

General Info

  • Alternative names
    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A
  • RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationSecreted; cell wall.

References for Natural Protein A (FITC) (ab7455)

ab7455 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"