Overview

Description

  • Nature
    Native
  • Source
    Native
  • Conjugation
    HRP

Specifications

Our Abpromise guarantee covers the use of ab7456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

    Immunomicroscopy

  • Form
    Liquid
  • Additional notes
    Molecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

General Info

  • Alternative names
    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A
  • Relevance
    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Secreted; cell wall.

References

This product has been referenced in:
  • Forstneric V  et al. Distinctive Recognition of Flagellin by Human and Mouse Toll-Like Receptor 5. PLoS One 11:e0158894 (2016). IP ; Human . Read more (PubMed: 27391968) »
  • Maity B  et al. Regulator of G Protein Signaling 6 (RGS6) Protein Ensures Coordination of Motor Movement by Modulating GABAB Receptor Signaling. J Biol Chem 287:4972-81 (2012). Read more (PubMed: 22179605) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry.

The best dilution of ab7456 for your application will depend on thethe individual experiment which will require some individual optimization. However, I can suggest dilutions of from 1:1000 and 1:5000 are usually ...

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Thank you for contacting us.

Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.


The links to the datasheets for protein A (HRP) and pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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