Overview

Description

  • NatureNatural
  • SourceNative
  • ConjugationHRP

Specifications

Our Abpromise guarantee covers the use of ab7456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

    Immunomicroscopy

  • FormLiquid
  • Additional notesMolecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

General Info

  • Alternative names
    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A
  • RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationSecreted; cell wall.

References for Natural Protein A (HRP) (ab7456)

This product has been referenced in:
  • Maity B  et al. Regulator of G Protein Signaling 6 (RGS6) Protein Ensures Coordination of Motor Movement by Modulating GABAB Receptor Signaling. J Biol Chem 287:4972-81 (2012). Read more (PubMed: 22179605) »
  • Bowick GC  et al. Identification of differentially activated cell-signaling networks associated with pichinde virus pathogenesis by using systems kinomics. J Virol 81:1923-33 (2007). Read more (PubMed: 17151108) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry.

The best dilution of ab7456 for your application will depend on thethe individual experiment which will require some individual optimization. However, I can suggest dilutions of from 1:1000 and 1:5000 are usually ...

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Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.


The links to the datasheets for protein A (HRP) and pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"