Native Protein G (Alkaline Phosphatase) (ab7461)
Key features and details
- Expression system: Native
- Purity: > 98% Protein G purified
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr
Description
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Product name
Native Protein G (Alkaline Phosphatase)
See all Protein G proteins and peptides -
Purity
> 98 % Protein G purified. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Streptococcus -
Predicted molecular weight
63 kDa
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Conjugation
Alkaline Phosphatase
Specifications
Our Abpromise guarantee covers the use of ab7461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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Form
Liquid -
Additional notes
Molecular Weight (protein G): 33,000 daltons
Extinction Coefficient: 1% at 280 nm is 9.5 (E0.1% of 9.5)
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
General Info
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Alternative names
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
see all -
Relevance
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Cell Wall and Secreted
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab7461 has been referenced in 1 publication.
- Zheng W & Yu A EZH2-mediated suppression of lncRNA-LET promotes cell apoptosis and inhibits the proliferation of post-burn skin fibroblasts. Int J Mol Med 41:1949-1957 (2018). PubMed: 29393360