Natural Protein G (Alkaline Phosphatase) (ab7461)

Overview

Description

  • NatureNatural
  • SourceNative
  • ConjugationAlkaline Phosphatase

Specifications

Our Abpromise guarantee covers the use of ab7461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

  • FormLiquid
  • Additional notesMolecular Weight (protein G): 33,000 daltons Extinction Coefficient: 1% at 280 nm is 9.5(E0.1% of 9.5)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

    0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2

General Info

  • Alternative names
    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationCell Wall and Secreted

References for Natural Protein G (Alkaline Phosphatase) (ab7461)

ab7461 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Here are the answers to your questions. 1) What is the format of this protein G (e.g Fc region only, albumin domain only) Answer: Recombinant Protein G containing IgG binding domain 2) Do you have any reference on ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"