Overview

Description

  • NatureNatural
  • SourceNative
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Specifications

Our Abpromise guarantee covers the use of ab7459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Flow Cytometry

    Immunomicroscopy

  • FormLyophilised
  • Additional notesMolecular Weight (protein G): 33,000 daltons Extinction Coefficient: 1% at 280 nm is 9.5(E0.1% of 9.5)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

  • ReconstitutionReconstitute product with 0.5 ml of deionized water (or equivalent).

General Info

  • Alternative names
    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationCell Wall and Secreted

References for Natural Protein G (FITC) (ab7459)

ab7459 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that the Protein G protein (FITC) ab7459, should be reconstituted in 500 µl of deionized water. This provides a 0.5 mg/ml solution of the protein in a buffer of 0.02M Potassium Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2. This ...

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I can confirm that I have forwarded your inquiry to my colleague, who is protein product manager at Abcam. She is now looking into the requested product line and will be contacting me with updates soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"