The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In Situ Hybridisation
Additional notesWarning: This product is irritating to eyes, respiratory system and skin, and may cause sensitization by inhalation.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with 1ml of 50mM Tris/HCI buffer (pH7.6).
Tritirachium alkaline proteinase
RelevanceProteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name.
Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.