Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Rabbit

Specifications

Our Abpromise guarantee covers the use of ab92786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab92786 is prepared from plasminogen by activation with immobilized Human uPA.
  • Form
    Liquid
  • Additional notes
    Plasmin will undergo rapid autoproteolysis in the absence of Protease inhibitors such as benzamidine, and should be used quickly after thawing before significant activity losses occur.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.1M HEPES, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Plasminogen
    • PLG
  • Relevance
    Plasmin is an enzyme formed in the circulating blood, and many other extracellular fluids, from the zymogen plasminogen. Plasminogen is a single-chain glycoprotein with 790 amino acid residues. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to the family of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. Plasmin acts as a proteolytic factor in a variety of processes other than fibrinolysis; including embryonic development, tissue remodelling, tumour invasion and inflammation; in ovulation it weakens the walls of the Graafian follicle. A Deficiency of plasmin may lead to thrombosis, as clots are not degraded adequately.
  • Cellular localization
    Secreted

Images

  • 10% SDS-PAGE
    Lane 1: Plasmin (Non-reduced)
    Lane 2: Plasmin (Reduced)

References

ab92786 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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