Native rat Antithrombin III/ATIII protein (ab93317)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
Description
-
Product name
Native rat Antithrombin III/ATIII protein
See all Antithrombin III/ATIII proteins and peptides -
Biological activity
ab93317 is 98% active by Thrombin titration. -
Purity
> 95 % SDS-PAGE.
ab93317 is purified using several chromatographic steps and is > 98% pure by SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
-
Species
Rat
-
Specifications
Our Abpromise guarantee covers the use of ab93317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Additional notes
Solubility: > 5 mg/mL
Spectrophotometric Data:
Ultraviolet: Absorbance (280nm) = 0.52 epsilon0.1% = 0.65 Concentration = 0.8 mg/mL
Previously labelled as Antithrombin III.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- ANT3_HUMAN
- Antithrombin
- Antithrombin III
see all -
Function
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
Tissue specificity
Found in plasma. -
Involvement in disease
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab93317 has not yet been referenced specifically in any publications.