Natural rat Antithrombin III protein (ab93317)

Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Rat

Associated products

Specifications

Our Abpromise guarantee covers the use of ab93317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    ab93317 is 98% active by Thrombin titration.
  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab93317 is purified using several chromatographic steps and is > 98% pure by SDS-PAGE.
  • Form
    Liquid
  • Additional notes
    Solubility: > 5 mg/mL
    Spectrophotometric Data: Ultraviolet: Absorbance (280nm) = 0.52 epsilon0.1% = 0.65 Concentration = 0.8 mg/mL
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, 1mM EDTA, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • ANT3_HUMAN
    • Antithrombin
    • Antithrombin III
    • Antithrombin-III
    • AntithrombinIII
    • AT 3
    • AT III
    • AT3
    • AT3D
    • ATIII
    • Heparin cofactor I
    • MGC22579
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1
    • Serine cysteine proteinase inhibitor clade C member 1
    • Serine proteinase inhibitor clade C member 1
    • Serpin C1
    • Serpin family C member 1
    • Serpin peptidase inhibitor clade C (antithrombin) member 1
    • SERPINC1
    • THPH7
    see all
  • Function
    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificity
    Found in plasma.
  • Involvement in disease
    Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt

Images

  • SDS-PAGE showing ab93317 at 58 kDa.
    Lane 1: Antithrombin III protein
    Lane 2: Antithrombin III protein + 3X Molar excess Thrombin

References

ab93317 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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