The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Chromatographically prepared from the euglobin fraction of pancreas, then twice crystallized.
Additional notesAfter reconstitution, aliquot and freeze remaining portion.
2.0 U/mg (One unit will hydrolyze 1 µmole of Suc-Ala-Ala-Ala-pNA per minute at pH 8.8 and 37°C.)
Assay: 5-10 µg in 0.1M Tris pH 8.8 at 37°C containing 0.2mM Suc-(Al)3-pNA.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Buffer: Salt free
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with 0.05M Na Acetate 0.2M NaCl pH 5.0 to 1 mg/ml or desired concentration.
Chymotrypsin like elastase family member 1
Chymotrypsin-like elastase family member 1
Elastase 1 pancreatic
FunctionActs upon elastin.
Tissue specificitySkin; not expressed in pancreas.
Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.