The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The activity of ab123831 is ~14 units/mg, where one unit is defined as the amount of protein required to bind 1.0 µg of D-biotin.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituent: 10% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute the powder with desired buffer, such as phosphate-buffered saline. The reconstituted solution will be stable for three months at 4°C if it is added with sodium azide to a final concentration of 2 mM or thimerosal to 0.02%. The reconstituted solution may also be divided into aliquots and stored at -20°C for long-term storage.
RelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
References for Natural Streptavidin protein (ab123831)
has not yet been referenced specifically in any publications.
Publishing research using ab123831? Please let us know so that we can cite the reference in this datasheet.
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