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Over 2800 high quality purified secondary antibodies validated in diverse applications such as western blot, immunohistochemistry, immunocytochemistry, ELISA and flow cytometry. Discover how Abcam can support your research.
Our guide will help you choose the most appropriate secondary antibody for your application.
Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.
Understand the difference between direct and indirect methods for immunofluorescence.
Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.
Alexa Fluor® 488 has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.
Biotin forms large complexes with avidin or streptavidin for signal amplification. Learn about the methods that use biotinylated antibodies.