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Secondary antibodies

Over 2800 high quality purified secondary antibodies validated in diverse applications such as western blot, immunohistochemistry, immunocytochemistry, ELISA and flow cytometry. Discover how Abcam can support your research.

Features

Secondary antibody selection guide

Our guide will help you choose the most appropriate secondary antibody for your application.

IRDye® conjugated secondary antibodies

Learn about infrared fluorescent WB advantages vs ECL, including detection of more than one protein in the same blot at the same time.

Direct vs indirect IF

Understand the difference between direct and indirect methods for immunofluorescence.

Alexa Fluor® secondary antibodies

Learn why our Alexa Fluor® conjugated secondaries are ideal for multi-color experiments.

Alexa Fluor® 488 conjugated antibodies

Alexa Fluor® 488  has improved photostability and brightness compared to fluorescein isothiocyanate (FITC). See comparison.

Biotinylated antibodies

Biotin forms large complexes with avidin or streptavidin for signal amplification. Learn about the methods that use biotinylated antibodies.

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IHC protocols

ICC/IF protocol

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