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Fusion protein, corresponding to cytoplasmic C terminal amino acids 1751-1946 of Human Nav1.7.
Our Abpromise guarantee covers the use of ab85015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 226 kDa.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immunohistochemical analysis of Mouse brain tissue labeling Nav1.7 with ab85015 at 1μg/ml.
ab85015 at 1/100 dilution staining Nav1.7 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used. Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.
ab85015 has not yet been referenced specifically in any publications.