Overview

  • Product nameAnti-Nav1.8 antibody
    See all Nav1.8 primary antibodies
  • Description
    Rabbit polyclonal to Nav1.8
  • Tested applicationsSuitable for: ICC, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide, corresponding to amino acids 1892-1956 of Nav1.8

Properties

Applications

Our Abpromise guarantee covers the use of ab63331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/4000.
WB 1/4000. Predicted molecular weight: 217 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionTetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
  • Tissue specificityExpressed in the dorsal root ganglia and sciatic nerve.
  • Involvement in diseaseEpisodic pain syndrome, familial, 2
  • Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
    Contains 1 IQ domain.
  • DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications
    Ubiquitinated by NEDD4L; which promotes its endocytosis.
    Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
  • Cellular localizationCell membrane. It can be translocated to the cell membrane through association with S100A10.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPN3 antibody
    • mPN3 antibody
    • Peripheral nerve sodium channel 3 antibody
    • Pn3 (gene name) antibody
    • PN3 antibody
    • SCN10A antibody
    • SCNAA_HUMAN antibody
    • Sensory neuron sodium channel antibody
    • Sns (gene name) antibody
    • SNS antibody
    • Sodium channel protein type 10 subunit alpha antibody
    • Sodium channel protein type X alpha subunit antibody
    • Sodium channel protein type X subunit alpha antibody
    • Voltage-gated sodium channel alpha subunit Nav1.8 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.8 antibody
    see all

Anti-Nav1.8 antibody images



  • Predicted band size : 217 kDa
    Western blot analysis. A single band of strong NaV1.8-like immunoreactivity at approximately 240 kDa in the membrane fraction of rat DRG is shown. Note that membrane fractions of rat whole brain, cerebellum, skeletal muscle, heart, superior cervical ganglia, liver and kidney showed no detectable immunoreactivity. Membrane extract from CHO-SNS22 (a cell line stably transfected with rat NaV1.8 cDNA) showed strong immunoreactivity at the same size with DRG. Non-transfected CHO cells did not show any bands.
  • Immunohistochemistry was performed on 2 week old rat lumber DRG sections. NaV1.8-like immunoreactivity can be detected only in small diameter (<30 um) neurons.

References for Anti-Nav1.8 antibody (ab63331)

This product has been referenced in:
  • Isensee J  et al. Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons. J Cell Sci 127:216-29 (2014). Read more (PubMed: 24190886) »

See 1 Publication for this product

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Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

The antibodies we have are; ab74095, ab63331, ab11...

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