The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/4000. Predicted molecular weight: 217 kDa.
Use at an assay dependent concentration.
FunctionTetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
Tissue specificityExpressed in the dorsal root ganglia and sciatic nerve.
Involvement in diseaseEpisodic pain syndrome, familial, 2
Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily. Contains 1 IQ domain.
DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
Post-translational modificationsUbiquitinated by NEDD4L; which promotes its endocytosis. Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Cellular localizationCell membrane. It can be translocated to the cell membrane through association with S100A10.
Predicted band size : 217 kDa Western blot analysis. A single band of strong NaV1.8-like immunoreactivity at approximately 240 kDa in the membrane fraction of rat DRG is shown. Note that membrane fractions of rat whole brain, cerebellum, skeletal muscle, heart, superior cervical ganglia, liver and kidney showed no detectable immunoreactivity. Membrane extract from CHO-SNS22 (a cell line stably transfected with rat NaV1.8 cDNA) showed strong immunoreactivity at the same size with DRG. Non-transfected CHO cells did not show any bands.