• Product nameAnti-Nav1.8 antibody
    See all Nav1.8 primary antibodies
  • Description
    Rabbit polyclonal to Nav1.8
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the C terminal domain of Nav1.8 (Human).



Our Abpromise guarantee covers the use of ab66743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/5000. Predicted molecular weight: 217 kDa.


  • RelevanceNav1.8 protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, Nav1.8 forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Nav1.8 plays a role in neuropathic pain mechanisms.
  • Cellular localizationIntegral membrane protein. Nav1.8 can be translocated to the extracellular membrane through association with S100A10.
  • Database links
  • Alternative names
    • mPN3 antibody
    • Peripheral nerve sodium channel 3 antibody
    • Pn3 (gene name) antibody
    • PN3 antibody
    • Scn10a antibody
    • Sensory neuron sodium channel antibody
    • Sns (gene name) antibody
    • SNS antibody
    • sodium channel protein type 10 subunit alpha antibody
    • Sodium channel protein type X alpha subunit antibody
    • Voltage-gated sodium channel alpha subunit Nav1.8 antibody
    see all

Anti-Nav1.8 antibody images

  • ab66743 staining Nav1.8 in human sperm cells by Immunocytochemistry/ Immunofluorescence.
    Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab66743 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.

References for Anti-Nav1.8 antibody (ab66743)

This product has been referenced in:
  • Cejudo-Roman A  et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013). Flow Cyt, ICC/IF ; Human . Read more (PubMed: 24086692) »
  • Pinto FM  et al. Molecular and functional characterization of voltage-gated sodium channels in human sperm. Reprod Biol Endocrinol 7:71 (2009). ICC/IF ; Human . Read more (PubMed: 19607678) »

See all 2 Publications for this product

Product Wall

Thank you for getting back to me. Thats no problem at all. If you are going to use ab66743, please be aware as discussed previously that we have not used it in IHC ourselves and cannot therefore guarantee that it would work. It would therefore no...

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Thank you for contacting us in regards to the anti-Nav1.8 antibodies ab66743, ab83936, ab93616. I am sorry for the delay in my reply, I have been seeking information in regards to the IHC protocols used with these antibodies.

The antibodies y...

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