Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
Expressed in the dorsal root ganglia and sciatic nerve.
Involvement in disease
Episodic pain syndrome, familial, 2
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily. Contains 1 IQ domain.
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
Ubiquitinated by NEDD4L; which promotes its endocytosis. Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
Cell membrane. It can be translocated to the cell membrane through association with S100A10.
Immunocytochemistry/ Immunofluorescence - Nav1.8 antibody (ab66743)Image from Pinto FM et al, Reprod Biol Endocrinol. 2009 Jul 16;7:71, Fig 2.
ab66743 staining Nav1.8 in human sperm cells by Immunocytochemistry/ Immunofluorescence. Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab66743 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.