Overview

  • Product nameAnti-NAV3 antibody
  • Description
    Rabbit polyclonal to NAV3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    QLCGSATSLEERPRA

    conjugated to KLH, corresponding to amino acids 1516-1530 of Human NAV3

  • Positive control
    • HEK-293T cell lysate expressing human NAV3.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab69868 is affinity-purified using the immunizing peptide immobilized on agarose.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 256 kDa.

Staining of the NAV3 band by immunoblotting is specifically inhibited by the NAV3 immunizing peptide.

Target

  • FunctionMay regulate IL2 production by T-cells. May be involved in neuron regeneration.
  • Tissue specificityHighly expressed in brain. Expressed at low levels in heart and placenta. Present in activated T-cells but not in resting T-cells (at protein level). Down-regulated in primary neuroblastoma.
  • Involvement in diseaseA chromosomal aberration disrupting NAV3 has been found in patients with Sezary syndrome (PubMed:16166283). Translocation t(12;18)(q21;q21.2) (PubMed:16166283).
  • Sequence similaritiesBelongs to the Nav/unc-53 family.
    Contains 1 CH (calponin-homology) domain.
  • Developmental stageExpressed in fetal brain.
  • Cellular localizationNucleus outer membrane.
  • Information by UniProt
  • Database links
  • FormThere are 3 isoforms produced by alternative splicing.
  • Alternative names
    • KIAA0938 antibody
    • NAV 3 antibody
    • NAV3 antibody
    • NAV3_HUMAN antibody
    • Neuron navigator 3 antibody
    • POMFIL1 antibody
    • Pore membrane and/or filament-interacting-like protein 1 antibody
    • Steerin 3 antibody
    • Steerin-3 antibody
    • STEERIN3 antibody
    • Unc 53 homolog 3 antibody
    • Unc-53 homolog 3 antibody
    • unc53H3 antibody
    see all

References for Anti-NAV3 antibody (ab69868)

This product has been referenced in:
  • Shioya M  et al. Aberrant microRNA expression in the brains of neurodegenerative diseases: miR-29a decreased in Alzheimer disease brains targets neuron navigator-3. Neuropathol Appl Neurobiol : (2010). WB ; Human . Read more (PubMed: 20202123) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK cell lysate)
Total protein in input 300 µg
Treatment Transfected with GFP-NAV3 plasmid
Immuno-precipitation step Other - Dynabeads-protein A magnetic beads
Specification HEK cell lysate
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Submitted Oct 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Scy-tek Superblock blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO ph9
Sample Human Tissue sections (Cerebellum)
Specification Cerebellum
Permeabilization No
Fixative Formaldehyde
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Verified customer

Submitted Jan 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"