Nb2 nuclear extract lysate (prolactin stimulated) (ab14869)

Overview

  • Product nameNb2 nuclear extract lysate (prolactin stimulated)
    See all Nb2 lysates
  • Description
    Nb2 nuclear extract lysate
  • General notesThe Nb2 nuclear extract derived from rat lymphocytes have been collected in Lysis Buffer after a 20 minute stimulation with 25 ng/ml of prolactin at 37°C immediately prior to harvesting. The protein content has been determined by a Bradford-based assay.

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • BackgroundThe Nb-2 rat lymphoma line was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Cells have a modified prolactin receptor and are used for in vitro bioassay of lactogenic hormones, studies of the mechanisms of lactogen induced mitogenesis, studies into the action of vinca alkaloids and studies of tumour progression.

References for Nb2 nuclear extract lysate (prolactin stimulated) (ab14869)

ab14869 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"