Overview

  • Product name
  • Description
    Rabbit polyclonal to NBL1
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from C-terminus of human NBL1.

  • Positive control
    • Human lung carcinoma tissue, HeLa cells, extracts from Jurkat and COLO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance
    NBL1 (Neuroblastoma, suppression of tumorigenicity 1) is a candidate tumor suppressor of neuroblastoma and may play a role in preventing cells from entering the final stage (G1/S) of the transformation process. NBL1 is a member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. It is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • D1S1733E antibody
    • DAN antibody
    • DAN domain family member 1 antibody
    • DAND 1 antibody
    • DAND1 antibody
    • Differential screening selected gene aberrant in neuroblastoma antibody
    • MGC8972 antibody
    • NB antibody
    • NBL 1 antibody
    • Neuroblastoma candidate region suppression of tumorigenicity 1 antibody
    • Neuroblastoma suppression of tumorigenicity 1 antibody
    • Neuroblastoma suppressor of tumorigenicity 1 antibody
    • NO 3 antibody
    • NO3 antibody
    • Zinc finger protein DAN antibody
    see all

Images

  • HeLa cells with ab70066 at 1/500 dilution, with and without the immunising peptide.
  • Human lung carcinoma tissue with ab70066 at 1/50 dilution, with and without the immunising peptide.
  • All lanes : Anti-NBL1 antibody (ab70066) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : COLO cell extract
    Lane 3 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 19 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Fessel JP  et al. Interaction between bone morphogenetic protein receptor type 2 and estrogenic compounds in pulmonary arterial hypertension. Pulm Circ 3:564-77 (2013). WB ; Mouse . Read more (PubMed: 24618541) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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