Overview

  • Product nameNBL1 Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 35 pg/ml
  • Range
    27.43 pg/ml - 20000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 111.3 107% - 122%
    Serum 100.1 91% - 109%
    Plasma 81.82 72% - 90%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s NBL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human NBL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NBL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NBL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm..

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionPossible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
  • Tissue specificityMost abundant in normal lung and meningioma.
  • Sequence similaritiesBelongs to the DAN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • DAN
    • DAN domain family member 1
    • DAND 1
    • DAND1
    • Differential screening selected gene aberrant in neuroblastoma
    • MGC8972
    • NB
    • NBL 1
    • NBL1
    • NBL1_HUMAN
    • Neuroblastoma candidate region suppression of tumorigenicity 1
    • Neuroblastoma suppression of tumorigenicity 1
    • Neuroblastoma suppressor of tumorigenicity 1
    • NO 3
    • NO3
    • Protein N03
    • Zinc finger protein DAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

NBL1 Human ELISA Kit images

  • Representative Standard Curve using ab99997

  • Representative Standard Curve using ab99997

Protocols

References for NBL1 Human ELISA Kit (ab99997)

ab99997 has not yet been referenced specifically in any publications.

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