Anti-NBR1 antibody (ab126175)
Key features and details
- Rabbit polyclonal to NBR1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NBR1 antibody
See all NBR1 primary antibodies -
Description
Rabbit polyclonal to NBR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 389-678 of Human NBR1 (UniProt Q14596).
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Positive control
- Jurkat whole cell lysate; 293T and A431 cell lysates; Human Breast Cancer tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 107 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. -
Sequence similarities
Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger. -
Domain
The OPR domain mediates interaction with SQSTM1. -
Cellular localization
Cytoplasm. Cytoplasmic vesicle > autophagosome. Lysosome. Cytoplasm > myofibril > sarcomere > M line. In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation. - Information by UniProt
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Database links
- Entrez Gene: 4077 Human
- Omim: 166945 Human
- SwissProt: Q14596 Human
- Unigene: 277721 Human
- Unigene: 708158 Human
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Alternative names
- 1A1 3B antibody
- 1A13B antibody
- B box protein antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab126175 has been referenced in 3 publications.
- Bensalem J et al. Measurement of autophagic flux in humans: an optimized method for blood samples. Autophagy 17:3238-3255 (2021). PubMed: 33164641
- Jo DS et al. Loss of HSPA9 induces peroxisomal degradation by increasing pexophagy. Autophagy 16:1989-2003 (2020). PubMed: 31964216
- Nguyen N et al. The insufficiency of ATG4A in macroautophagy. J Biol Chem 295:13584-13600 (2020). PubMed: 32732290