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Rabbit polyclonal to NBR1
Recombinant fragment, corresponding to a region within amino acids 389-678 of Human NBR1 (UniProt Q14596).
Jurkat whole cell lysate; 293T and A431 cell lysates; Human Breast Cancer tissue
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.
Contains 1 OPR domain.
Contains 1 UBA domain. Contains 1 ZZ-type zinc finger.
The OPR domain mediates interaction with SQSTM1.
Cytoplasm. Cytoplasmic vesicle > autophagosome. Lysosome. Cytoplasm > myofibril > sarcomere > M line. In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation.
Information by UniProt
1A1 3B antibody
B box protein antibody
Western blot - Anti-NBR1 antibody (ab126175)
Anti-NBR1 antibody (ab126175) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 107 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NBR1 antibody (ab126175)
ab126175, at 1/250 dilution, staining NBR1 in paraffin-embedded Human Breast cancer tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"