Overview

  • Product nameAnti-NBR1 antibody
    See all NBR1 primary antibodies
  • Description
    Mouse monoclonal to NBR1
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: EPQVTLNVTF KNEIQSFLVS DPENTTWADI EAMVKVSFDL NTIQIKYLDE ENEEVSINSQ GEYEEALKMA VKQGNQLQMQ VHEGHHVVDE APPPV, corresponding to amino acids 2-97 of Human NBR1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 23626693
IHC-P Use a concentration of 4 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionActs probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.
  • Sequence similaritiesContains 1 OPR domain.
    Contains 1 UBA domain.
    Contains 1 ZZ-type zinc finger.
  • DomainThe OPR domain mediates interaction with SQSTM1.
  • Cellular localizationCytoplasm. Cytoplasmic vesicle > autophagosome. Lysosome. Cytoplasm > myofibril > sarcomere > M line. In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1A1 3B antibody
    • 1A13B antibody
    • Cell migration-inducing gene 19 protein antibody
    • KIAA0049 antibody
    • M17S2 antibody
    • Membrane component chromosome 17 surface marker 2 antibody
    • MIG 19 antibody
    • MIG19 antibody
    • Migration inducing protein 19 antibody
    • NBR 1 antibody
    • Nbr1 antibody
    • NBR1_HUMAN antibody
    • Neighbor of BRCA1 gene 1 antibody
    • Neighbor of BRCA1 gene 1 protein antibody
    • Next to BRCA1 gene 1 protein antibody
    • Ovarian carcinoma antigen CA125 antibody
    • Protein 1A1-3B antibody
    see all

Anti-NBR1 antibody images

  • Overlay histogram showing HeLa cells stained with ab55474 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55474, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot against tagged recombinant protein immunogen using ab55474 NBR1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

  • ab55474 (4µg/ml) staining NBR1 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is moderate cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-NBR1 antibody (ab55474)

This product has been referenced in:
  • Cunningham DL  et al. Novel binding partners and differentially regulated phosphorylation sites clarify Eps8 as a multi-functional adaptor. PLoS One 8:e61513 (2013). WB, IP ; Human . Read more (PubMed: 23626693) »
  • Manzanillo PS  et al. The ubiquitin ligase parkin mediates resistance to intracellular pathogens. Nature 501:512-6 (2013). Read more (PubMed: 24005326) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 10 µg
Specification 293T
Gel Running Conditions Reduced Denaturing (4-12%)
Username

Dr. Faraz Mardakheh

Verified customer

Submitted Dec 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"