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This antibody was raised against a small cell lung carcinoma specimen.
Our Abpromise guarantee covers the use of ab9272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||1/500 - 1/1000.|
|IHC-P||1/500 - 1/1000.|
|WB||Use at an assay dependent concentration.|
|IHC (PFA fixed)||1/500 - 1/1000.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab9272 at a dilution of 1/1000, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).