Overview

  • Product nameAnti-NCAM antibody [123C3]
    See all NCAM primary antibodies
  • Description
    Mouse monoclonal [123C3] to NCAM
  • SpecificityThis antibody stains neural cell adhesion molecule (NCAM) which is present in small cell lung cancer and lung carcinoids. It also reacts with a number of non-small cell lung carcinomas and neuroendocrine and neuronal derived tissues. In addition, it is internalized after binding to its antigen on SCLC cell lines, making it an excellent reagent for tumor imaging in xenograft models. ab9272 reacts with all three isoforms of NCAM.
  • Tested applicationsSuitable for: IHC-FrFl, ICC, IHC-Fr, IHC-P, WB, IHC (PFA fixed), Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human, Zebrafish
  • Immunogen

    This antibody was raised against a small cell lung carcinoma specimen.

  • EpitopeThis antibody recognizes an epitope in the NCAM exons 11-13 which is dependent on an intact conformation of the first fibronectin type-III homologous domain encoded by these exons.

Properties

Applications

Our Abpromise guarantee covers the use of ab9272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/1000.
IHC-P 1/500 - 1/1000.
WB Use at an assay dependent concentration.
IHC (PFA fixed) 1/500 - 1/1000.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThis protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similaritiesContains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Anti-NCAM antibody [123C3] images

  • ab9272 at a dilution of 1/1000, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing hippocampal neuron body and processes: [A] 20x objective and [B] punctate cytoplasmic staining; 40x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
  • Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
  • Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9272 reacts with developing neuronal tissue (strong) and muscle. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
  • Immunostaining of the neural cell adhesion molecule (NCAM) in small cell lung cancer cell line NCI-H82
  • Overlay histogram showing SH-SY5Y cells stained with ab9272 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9272, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-NCAM antibody [123C3] (ab9272)

This product has been referenced in:
  • Kong Q  et al. Effects of pharmacological treatments on hippocampal NCAM1 and ERK2 expression in epileptic rats with cognitive dysfunction. Oncol Lett 12:1783-1791 (2016). IHC-P ; Rat . Read more (PubMed: 27588125) »
  • Wagner M  et al. Selective expansion of the {beta}-cell compartment in the pancreas of keratinocyte growth factor transgenic mice. Am J Physiol Gastrointest Liver Physiol : (2008). Read more (PubMed: 18372394) »

See all 10 Publications for this product

Product Wall


The ability of ab9272 to recognize the nCAM epitope is dependent on the intact conformation of the first fibronectin type III domain (human nCAM aa506-605). The homology of 93.4% between human (http://www.uniprot.org/uniprot/P13591) and hamster (h...

Read More
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (fibroblast)
Loading amount 100 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 15 2012

Application Western blot
Sample Zebrafish Cell lysate - whole cell (brain and whole fish lysate)
Loading amount 100 µg
Specification brain and whole fish lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Dec 08 2011

Thank you for your enquiry. Unfortunately, no specific immunogen sequence is known, since the antibody was raised against a membrane preparation of SCLC and not against a specific amino acid sequence.

Thank you for your enquiry. Following correspondence with the lab I have determined that both ab9018 and ab9272 react against all three isoforms of NCAM. I hope this information helps, please do not hesitate to contact us if you need any more ad...

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Thank you for your enquiry. Ab8077 recognises NCAM-140 and 180 we do not have any information relating to NCAM 120. I am still in the process of sourcing further information regarding Ab9018 and Ab9272 and will be in touch with you again when I have an...

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Thank you for your enquiry and for your patience. I took a look at the NCAM antibodies that we currently have, and it does not appear that any of them recognize all three major isoforms. For example, ab8077, clone 123C3, recognizes a transmembrane glyc...

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