Overview

  • Product name
  • Description
    Goat polyclonal to NCAM
  • Specificity
    ab118291 is expected to recognize reported isoform 2 (NP_851996.2) only.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    KASWTRPEKQETLD

    with a Cysteine residue linker, corresponding to internal sequence amino acids 353-366 of Human NCAM (NP_851996.2).

  • Positive control
    • Human brain cerebellum, cerebral and frontal cortex lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab118291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 94 kDa). The observed molecular weight corresponds to the glycosylated form.

Target

  • Function
    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similarities
    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Anti-NCAM antibody images

  • Anti-NCAM antibody (ab118291) at 0.01 µg/ml + Human frontal cortex lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 94 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)

References for Anti-NCAM antibody (ab118291)

ab118291 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Human Tissue sections (mouse brain)
Specification
mouse brain
Username

Abcam user community

Verified customer

Submitted Jan 19 2016

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain
Username

Abcam user community

Verified customer

Submitted Dec 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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