Anti-NCAM antibody [EP2567Y] (ab75813)


  • Product name
    Anti-NCAM antibody [EP2567Y]
    See all NCAM primary antibodies
  • Description
    Rabbit monoclonal [EP2567Y] to NCAM
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NCAM aa 800 to the C-terminus (C terminal).

  • Positive control
    • WB: SH-SY5Y cell lysate. ICC/IF: SH-SY5Y cells. Flow Cyt: SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab75813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/100000. Detects a band of approximately 180 kDa (predicted molecular weight: 93 kDa).
IP 1/100.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.


  • Function
    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similarities
    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Anti-NCAM antibody [EP2567Y] images

  • ab75813 staining NCAM in human adipose cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab75813 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75813, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab75813 at 1/100 dilution staining NCAM in SH-SY5Y cells by Immunofluorescence.
  • Anti-NCAM antibody [EP2567Y] (ab75813) at 1/100000 dilution + SH-SY5Y cell lysate at 10 µg

    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 93 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)

References for Anti-NCAM antibody [EP2567Y] (ab75813)

This product has been referenced in:
  • Espuny-Camacho I  et al. Hallmarks of Alzheimer's Disease in Stem-Cell-Derived Human Neurons Transplanted into Mouse Brain. Neuron 93:1066-1081.e8 (2017). ICC/IF ; Human . Read more (PubMed: 28238547) »
  • Taromi S  et al. An orthotopic mouse model of small cell lung cancer reflects the clinical course in patients. Clin Exp Metastasis 33:651-60 (2016). IHC ; Human . Read more (PubMed: 27380917) »

See all 8 Publications for this product

Product Wall

Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Human Cell (Adipose tissue)
Adipose tissue
Yes - 0.3% TritonX-100

Abcam user community

Verified customer

Submitted Mar 16 2015

Because this rabbit monoclonal antibody product is produced from 293 cells, the Fc part of Ab will be glycosylated automatically.


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