Recombinant
RabMAb

Anti-NCAM antibody [EP2567Y] (ab75813)

Overview

  • Product name
    Anti-NCAM antibody [EP2567Y]
    See all NCAM primary antibodies
  • Description
    Rabbit monoclonal [EP2567Y] to NCAM
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NCAM aa 800 to the C-terminus (C terminal).

  • Positive control
    • WB: SH-SY5Y cell lysate. ICC/IF: SH-SY5Y cells. Flow Cyt: SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab75813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/100000. Detects a band of approximately 180 kDa (predicted molecular weight: 93 kDa).
IP 1/100.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similarities
    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Anti-NCAM antibody [EP2567Y] images

  • ab75813 staining NCAM in human adipose cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab75813 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75813, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab75813 at 1/100 dilution staining NCAM in SH-SY5Y cells by Immunofluorescence.
  • Anti-NCAM antibody [EP2567Y] (ab75813) at 1/100000 dilution + SH-SY5Y cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 93 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)

References for Anti-NCAM antibody [EP2567Y] (ab75813)

This product has been referenced in:
  • Espuny-Camacho I  et al. Hallmarks of Alzheimer's Disease in Stem-Cell-Derived Human Neurons Transplanted into Mouse Brain. Neuron 93:1066-1081.e8 (2017). ICC/IF ; Human . Read more (PubMed: 28238547) »
  • Taromi S  et al. An orthotopic mouse model of small cell lung cancer reflects the clinical course in patients. Clin Exp Metastasis 33:651-60 (2016). IHC ; Human . Read more (PubMed: 27380917) »

See all 8 Publications for this product

Product Wall

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Human Cell (Adipose tissue)
Specification
Adipose tissue
Permeabilization
Yes - 0.3% TritonX-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 16 2015

Because this rabbit monoclonal antibody product is produced from 293 cells, the Fc part of Ab will be glycosylated automatically.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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