Overview

  • Product nameAnti-NCAM antibody [MEM-188]
    See all NCAM primary antibodies
  • Description
    Mouse monoclonal [MEM-188] to NCAM
  • SpecificityCD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested. Positive membrane-specific staining was observed in the paraprostatic autonomic ganglion.
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Tissue/ cell preparation (Human). (KG-1 acute myelogenous leukaemia cell line).

  • Positive control
    • paraprostatic autonomic ganglion

Properties

Applications

Our Abpromise guarantee covers the use of ab8233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Use standard ABC/DAB+ staining technique.
Flow Cyt Use a concentration of 0.5 - 3 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

Target

  • FunctionThis protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similaritiesContains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

Anti-NCAM antibody [MEM-188] images

  • ab8233 staining NCAM in human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed (sections were incubated with 0.5g/L of Trypsin in PBS for 30 minutes at 25ºC prior to blocking). Samples were then blocked using 5% serum for 16 hours at 4°C, then incubated with ab8233 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated rabbit anti-mouse polyclonal at a 1/300 dilution.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab8233 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-NCAM antibody [MEM-188] (ab8233)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for contacting us. Although we have tested in mouse cells for FACS, we have not tesed in live cells and cannot guarantee its use for this purpose. Please let me know if you have any further questions.

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Je suis ravi que vous souhaitiez participer à notre offre et tester ab8233 en ICC/IF. Le code promotionnel correspondant est *******, il est valable 4 mois et pour la c...

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Merci de votre intérêt pour ab8233.

Comme expliqué par téléphone, cet anticorps n’a pas encore été testé en ICC/IF et nous n’offrons pas d’échantillon gratuit &ag...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (Axillary Lymph node)
Specification Axillary Lymph node
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 28 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Formaldehyde
Antigen retrieval step Enzymatic
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 28 2011

Thank you for your enquiry and patience. Looking through the NCAM antibodies that we currently have, it does not appear that there is one that specifically reacts with the 180 kDa isoform and is known not to cross-react with other isoforms. If you h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"