Anti-NCAM antibody [MEM-188] (Allophycocyanin) (ab28335)

Overview

  • Product nameAnti-NCAM antibody [MEM-188] (Allophycocyanin)
    See all NCAM primary antibodies
  • Description
    Mouse monoclonal [MEM-188] to NCAM (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Specificityab28335 recognises an isoform of NCAM characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KG-1 human acute myelogenous leukaemia cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab28335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells. Use 20 µl per 100 µl of whole blood.



ab91364-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThis protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similaritiesContains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

References for Anti-NCAM antibody [MEM-188] (Allophycocyanin) (ab28335)

ab28335 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll-Hypaque gradient
Sample buffer: PBS
Permeabilization No
Gating Strategy lymphocytes
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Verified customer

Submitted Apr 24 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry these 2 products did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you very much for contacting us with your question.

We do expectall antibodies from the same clone tohave the same cross-reactivity, so Ab25640 should react with mouse samples despite the difference in conjugation between it and Ab28335...

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Thank you for your call today and for letting us know about this issue.

As we discussed, please keep me updated with the results after using positive and negative control cell lines. I will also try to find any additional information that mig...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"