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This antibody was raised against the small lung cancer cell line NCI-H82.
Our Abpromise guarantee covers the use of ab9018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells. (paraformaldehyde or methanol fixed cells)
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||1/100 - 1/200. see detailed protocol|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|WB||1/100 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
Immunocytochemical analysis of Adult-Derived Human Liver Stem Cells (ADHLSC) in the left image and Hepatic Stellate Cells (HSC) in the right image. ab9018 was used to label NCAM, which is expressed in the HSC. Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. Blocking was with 3.3% hydrogen peroxide for 3 minutes. Permeabilization with D-PBS and 1% Triton X-100 for 10 minutes. Cells incubated with ab9018 at 1/100 for 1 hour. DAB staining. Counterstaining with Mayer's hematoxylin.
ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30
NB: We recommend indirect amplification of immunofluorescence for ab9018
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).