This antibody was raised against the small lung cancer cell line NCI-H82.
Our Abpromise guarantee covers the use of ab9018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells. (paraformaldehyde or methanol fixed cells)
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||1/100 - 1/200. see detailed protocol|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|WB||1/100 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30
NB: We recommend indirect amplification of immunofluorescence for ab9018
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).