Overview

  • Product nameAnti-NCAPG2 antibody
    See all NCAPG2 primary antibodies
  • Description
    Rabbit polyclonal to NCAPG2
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between C terminal residues 1100 -1143 of human NCAPG2. (AAH43404.1)

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 1-4 µg/mg lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceNCAPG2 is 1 of 3 non-SMC subunits that define condensin II. Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMC subunits that defines condensin II.
    • Cellular localizationnucleus
    • Database links
    • Alternative names
      • CAP G2 antibody
      • Condensin 2 complex subunit G2 antibody
      • FLJ20311 antibody
      • hCAP G2 antibody
      • Leucine zipper protein 5 antibody
      • More than blood homolog antibody
      • MTB antibody
      • NCAPG 2 antibody
      • Non SMC condensin II complex subunit G2 antibody
      • Non SMC condensin II complex, subunit G2 antibody
      see all

    Anti-NCAPG2 antibody images

    • All lanes : Anti-NCAPG2 antibody (ab70350) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg


      Predicted band size : 131 kDa
      Observed band size : 131 kDa

    References for Anti-NCAPG2 antibody (ab70350)

    This product has been referenced in:
    • Liu W  et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB ; Human . Read more (PubMed: 20622854) »
    • Centore RC  et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell 40:22-33 (2010). WB ; Human . Read more (PubMed: 20932472) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Non-reduced Denaturing
    Sample Mouse Cell lysate - whole cell (Mouse fetal liver cells)
    Specification Mouse fetal liver cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

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    Verified customer

    Submitted Sep 09 2013

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